The two primary steps of biopharmaceutical production processes—cell line development and engineering (CLD&E) and culture procedures—undergo constant improvement, with the main goal being to create efficient processes and high-yielding expression hosts to boost revenues.
In terms of process development, major advancements have been made in the media and CHO cell-culture compositions to hasten cellular growth and sustain high viable cell densities (VCD) for an extended period of time. Similar to this, at various levels of CLD&E, the functionality of CHO host cells has also been improved, from the high expression of therapeutic protein to its regular secretion out of the cells. Additionally, CHO cells are created to meet particular standards for product quality, such as precise N-glycosylation modifications and the deletion or reduction of sequence variations in therapeutic proteins. Cellular engineering has recently been used to create CHO cell lines with stability guarantees and sustained high titers even in continuous growth.
Now that the CLD process has been automated and high-throughput technologies have been integrated, we can quickly produce a large number of new clones, producing a tonne of data for their analysis. These methods are nevertheless still illogical and empirical. Recent developments in high-throughput omics data profiling and the availability of systematic modelling frameworks have made it possible to obtain engineering targets in a reasoned manner based on background knowledge of CHO cells.
Due to modern CLD platforms and adaptable cellular engineering approaches, the manufacturing yields of CHO cell lines have drastically grown during the previous three decades. Traditionally, the development of CHO cell lines has primarily relied on broad cell screening of mutants produced at random and evaluation of those mutations. Future genome engineering of cellular targets is now possible thanks to the current availability of the CHO genome, which is precise and targeted.
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